Morphological characterization of Artemia salina in its different developmental cycles and its application in biocompatibility testing
Keywords:
Artemia Salina, characterization, toxicity, morphology, feeding, protocolArticle Sidebar
Accepted 2025-10-01
Published 2025-10-01
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Background: Artemia Salina is a tiny crustacean that can tolerate high salt concentrations and survive in low-oxygen conditions. It is sexually dimorphic, meaning it has both sexual and asexual life cycles. In its adult stage, it measures between 17-18 mm in length and is used in aquaculture and toxicity testing.
Objective: To determine the most suitable stage of development of Artemia Salina for biocompatibility testing.
Materials and methods: An in vitro experimental study was conducted using Artemia salina as a biomodel. Detailed observations were made of the life cycle of Artemia salina, from its cyst stage to adulthood.
Results: A culture and feeding protocol was established for its maintenance in the laboratory. Common dental biomaterials were used to evaluate their cytotoxicity in Artemia larvae.
Conclusion: Toxicity tests with dental biomaterials showed that both calcium hydroxide and zinc oxide-eugenol were toxic to Artemia larvae, with a mortality rate of 100% within the first 24 hours.
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