Microbiología de las lesiones apicales crónicas supurativas con fístula

Abstract

The microbiology of24 teeth with Suppurative Chronic Apical Periodontitis and fistula lesions were identified. F orty eight samples were taken from adult patients that attended to the Dental School atthe Universidad del Valle, Cali-Colombia. Root canal samples were taken after crown drilling and disinfection which inc1uded also the adjacentmucous ofthe fistulous tract. Root canal samples were recovered with a 08 Maillefer ® sterile file overstepping 2mm in the apicallesion. Fistula samples were recovered using # 15 Meta Dental Co ® sterile paper points. Both, fistula and root canal samples were transfered to VMGA III transport media and processed at least 24 hours after sampling. Plating took place at the Oral Microbiology laboratory using selective media such as TSVB and non-selective media such as BBL plates supplemented with 5% sheep bloodlhemin adnmenadione. Anaerobic and facultative culture were incubated for 5 days. Microbiological determination was done using stereostopic microscopy for microbial colony morphology and rapid biochemical test. This study identified eighteen bacteria species. The more frequent species in root canals were: Streptococcus anaerobios and Fusobacterium sp (20.8%) 5 cases, Prevotella intermedia and Peptostreptococcus micros (16. 7%) 4 cases, Eubacterium sp and Gram (­) enteric rods (12.5%) 3 cases. The more frequent species in the fistula samples were: Porphyromonas gingivalis (33.3%) 8 cases, Streptococcus anaerobios (29.2%) 7 cases, Peptostreptococcus micros (25%) 6 cases. According to this results, the presence of pathogenic bacterias in the periapieallesion and in the fistulous traet may be important beeause the determination of endodontic mierobiolgy would be useful to eomplement the meehanieal therapy with antibiotic treatment.