In vitro effect of platelet rich plasma on periodontal ligament cells
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Summary: Introduction: Gingivitis and
Periodontitis are the common inflammatory diseases in our community. The reaction of the periodontal
tissues results of the interaction of the hosp defense at the biofilm formed around the teeth and
the gingival tissue. The severity of this entity depend of the composition of biofilm and local or
systemic factors can promote the bacterial colonization or behavioral components like tobacco and
stress can be modified the individual response. Interaction between defense response by hosp and
evasion mechanism by periodontopathogens plays an important role in the pathological process of
alveolar bone destroy and migration the junction epithelium. The periodontal therapy purpose is
arrest the disease progression. Recently in an attempt to increase the succes rate in regenerative
procedures, it has incorporated the use of platelet-rich plasma. It involves the growth factors that
are proposed as enhancers of tissue regeneration of the periodontal tissues. Purpose: The purpose of
the study was to evaluate the response of periodontal fibroblasts (PF) at different concentration of
platelet rich plasma in vitro. Metodology: Cellular cultures of PF were obtained from In Vitro
Research Center and PRP was get from the Hemocentro of the Red Cross, and dilutions were made to
obtain concentrations to 10, 15 and 20%. They were compared with 2 controls of 5% bovine fetal serum
(BFS) and fibroblastic growth factor (FGF). In order to determine the metabolic activity of the FP
stimulated by PRP, Kit XTT® was used. Results: Findings suggest that a higher concentration of PRP
results in positive effects on cellular growth and significantly increase in proliferation of FP at
concentration of 20%. Conclusions: PRP resulted in positive effects on periodontal fibroblasts. Key
words: Growth factor. Platelet -derived. Growth factor. Periodontal ligament. Fibroblasts. Guided
tissue regeneration. Platelet rich plasma. Healing.
Periodontitis are the common inflammatory diseases in our community. The reaction of the periodontal
tissues results of the interaction of the hosp defense at the biofilm formed around the teeth and
the gingival tissue. The severity of this entity depend of the composition of biofilm and local or
systemic factors can promote the bacterial colonization or behavioral components like tobacco and
stress can be modified the individual response. Interaction between defense response by hosp and
evasion mechanism by periodontopathogens plays an important role in the pathological process of
alveolar bone destroy and migration the junction epithelium. The periodontal therapy purpose is
arrest the disease progression. Recently in an attempt to increase the succes rate in regenerative
procedures, it has incorporated the use of platelet-rich plasma. It involves the growth factors that
are proposed as enhancers of tissue regeneration of the periodontal tissues. Purpose: The purpose of
the study was to evaluate the response of periodontal fibroblasts (PF) at different concentration of
platelet rich plasma in vitro. Metodology: Cellular cultures of PF were obtained from In Vitro
Research Center and PRP was get from the Hemocentro of the Red Cross, and dilutions were made to
obtain concentrations to 10, 15 and 20%. They were compared with 2 controls of 5% bovine fetal serum
(BFS) and fibroblastic growth factor (FGF). In order to determine the metabolic activity of the FP
stimulated by PRP, Kit XTT® was used. Results: Findings suggest that a higher concentration of PRP
results in positive effects on cellular growth and significantly increase in proliferation of FP at
concentration of 20%. Conclusions: PRP resulted in positive effects on periodontal fibroblasts. Key
words: Growth factor. Platelet -derived. Growth factor. Periodontal ligament. Fibroblasts. Guided
tissue regeneration. Platelet rich plasma. Healing.
Clavijo, G. M., Pinillos, P. A., & Gutiérrez, Óscar. (2007). In vitro effect of platelet rich plasma on periodontal ligament cells. Revista Estomatología, 15(1). https://doi.org/10.25100/re.v15i1.5604
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