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Background: Some procedures that are performed during the daily dental practice might favor environmental contamination by microorganisms. Therefore, their presence in materials that are routinely used in the dental office constitutes a risk of transmitting pathogen agents, thereby causing cross-contamination.


Aim: To evaluate microbial contamination of light-curing composites previously used in patients at the School of Dentistry, Universidad Cooperativa de Colombia, Envigado campus.
Materials and Methods: The experimental group consisted of 46 composite samples extracted from previously-used composite syringes. Composite from two as-received syringes and two sterile toothpicks were used as the control group. Samples were deposited in BHI broth for 48 hours at 37°C. After this time, a turbidity reading was performed. Contaminated samples were seeded in solid culture media and colonies were described according to their macro and microscopic characteristics. The SPSS 21.0 statistical software was used and Chi-square coefficient was calculated.
Results: 34.8% of composites were contaminated by Staphylococcus sp and Gram positive bacillus. No statistical significant differences between composite contamination and frequency of use were observed (p>0,05). Conclusions: Composite microbial contamination after a single use was demonstrated. Therefore, composite remnants contained in previously-used syringes might be responsible for cross-contamination.

Claudia-María Bedoya-Correa, Facultad de Odontología Universidad Cooperativa de Colombia. Envigado, Colombia.

Bióloga Especialista en Microbiología Ambiental, Docente.

Ángela-María Sarrazola-Moncada, Facultad de Odontología Universidad de Antioquia. Medellín, Colombia.

Docente de cátedra

Sandra-Milena Palacio-Espinosa, Facultad de Odontología Universidad Cooperativa de Colombia. Envigado, Colombia.

Odontóloga Magister en Epidemiología, Docente.

Olga-Lucia Julio-Madrid, Facultad de Odontología Universidad Cooperativa de Colombia. Envigado, Colombia.

Odontólogo

Natalia-Andrea Osorio-Rojo, Facultad de Odontología Universidad Cooperativa de Colombia. Envigado, Colombia.

Odontólogo

Astrid-Julieth Garzón-Henao, Facultad de Odontología Universidad Cooperativa de Colombia. Envigado, Colombia.

Odontólogo

Bedoya-Correa, C.-M., Sarrazola-Moncada, Ángela-M., Palacio-Espinosa, S.-M., Julio-Madrid, O.-L., Osorio-Rojo, N.-A., & Garzón-Henao, A.-J. (2016). Evaluation of microbial contamination in the light-curing composite used by dental students in their clinical practices. Revista Estomatología, 24(1), 24–29. https://doi.org/10.25100/re.v24i1.5793
1. Angelillo IF, Bianco A, Nobile CG, Pavia M. Evaluation of the efficacy of glutaraldehyde and peroxygen for disinfection of dental instruments. Lett Appl Microbiol. 1998; 27(5):292-6.
2. Shah AH, Wyne AH. Cross - infection control in dentistry: a review. Pakistan Oral & Dental Journal. 2010; 30(1):168-174.
3. Matsuyama M, Usami T, Masuda K, Niimi N, Ohta M, Ueda M. Prevention of infection in dental procedures. J Hosp Infect. 1997; 35(1):17-25.
4. Monarca S, Grottolo M, Renzi D, Paganelli C, Sapelli P, Zerbini I, et al. Evaluation of environmental bacterial contamination and procedures to control cross infection in a sample of Italian dental surgeries. Occup Environ Med. 2000; 57(11):721-6.
5. Gutiérrez S, Dussán D, Leal S, Sánchez A. Evaluación microbiológica de la desinfección en unidades odontológicas (estudio piloto). Rev Colomb Cienc Quím Farm [revista en línea] 2008 [fecha de acceso 22 de julio de 2015]; 37(2): 133-49 Disponible en: http://www.ciencias.unal.edu.co/unciencias/data-file/farmacia/revista/V37N2-03.pdf
6. Oliveira M, Barreto RM, Salgado IO, Chaves HD, Diniz CG. Avaliação da contaminação bacteriana em resinas compostas utilizadas nas clínicas de graduação da FO-UFJF. Odontol. Clín.-Cient. 2010; 9(1):73-6.
7. Taveira C, Máximo A, Souza J, Moreira F, Lopes L. Avaliação do controle de infecção de espátulas para resinas compostas - Um estudo com cirurgiões dentistas da cidade de Goiânia-GO. Rev Odontol Bras Central. 2010; 18(48):38-41.
8. Liébana Ureña J. Microbiología oral. 2° edición. Madrid: editorial McGrawHill-Interamericana de España, S.A.; 2002.
9. Mouton C, Robert JC. Bacteriología bucodental. 1° edición. Barcelona: editorial Masson, S.A.; 1995.
10. Szymańska J. Microbiological risk factors in dentistry. Current status of knowledge. Ann Agric Environ Med. 2005; 12(2): 157-63.
11. República de Colombia. Ministerio de salud. Resolución número 008430 de 1993. Por la cual se establecen las normas científicas, técnicas y administrativas para la investigación en salud [internet] 1993 [fecha de acceso 27 de agosto de 2015] Disponible en: https://www.invima.gov.co/images/pdf/medicamentos/resoluciones/etica_res_8430_1993.pdf
12. Wer l e S B , S a n t o s R C , D o t t o P P. Contaminação das resinas compostas em clínicas de instituição de ensino. Pesq Bras Odontoped Clin Integr. 2012; 12(4): 473-76.
13. Del Valle SC. Normas de bioseguridad en el consultorio odontológico. Acta odontol venez [revista en línea] 2002 [fecha de acceso 31 de agosto de 2015]; 4 0 ( 2 ) D i s p o n i b l e e n : h t t p : / / w w w.actaodontologica.com/ediciones/2002/2/
Volumen 24 Nº 1 201629n o r m a s _ b i o s e g u r i d a d _ c o n s u l t o r i o _odontologico.asp
14. Almeida JC, Prado AK, Silva WC, Pedrosa S, Moura MA, Chaves RM, et al. Contaminação de Resinas Compostas em Consultórios Odontológicos. Rev Odontol Bras Central. 2010; 19(50):211-15.
15. Cardoso CT, Pinto JR, Pereira EA, Barros LM, Freitas AB. Contaminação de Tubos de Resina Composta Manipulados sem Barreira de Proteção. Rev Odontol Bras Central. 2010; 18(48):71-5.
16. Madigan MT, Martinko JM, Dunlap PV, Clark DP. Brock. Biología de los Microorganismos. 12° edición. Madrid: editorial Prentice-Hall; 2009.
17. Secretaría distrital de salud, Bogotá, D.C. institución universitaria colegios d e C o l o m b i a , U N I C O C - C o l e g i o Odontológico. Guía de práctica clínica en salud oral [libro en línea] 2010 [fecha de acceso 10 de septiembre de 2015] Disponible en: http://www.disanejercito.mil.co/?idcategoria=27465&download=Y