Cultivo y caracterización in vitro de una línea celular de fibroblastos gingivales humanos
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The main goal is to establish a cellular line of fibroblast which come from human gingival tissue, identifying its morphologic features.
Samples were taken from gum of healthy patients without medical antecedents.
Three females and three male were selected. Ages were between 16 and 30 years old. A sample of 0,5 cm2 of gum was obtained to take it to a cellular growth enviroment. Cultures were incobated at 37°C, CO2 atmosphere at 5% for ten (10) days. Samples were observed daily with inverted light Microscope Olimpus II. At 6th day growth of fusiform cells was observed; their morphology was in coincidence with that of the fibroblasts. At 18th day a monolayer of 100% purity was observed. The cellular line was preserved and stored by freezing in liquid nitrogen.
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